2024, Vol. 5, Issue 2, Part A
Development and validation of spectrophotometric method for assay of Di[RS]-3-[4-(2-methoxy ethyl)phenoxy-1-(Isopropyl amino) propan-2-ol] tartrate (Metoprolol tartrate) by using 2, 3- dimethoxy stricychnidin-10-one-Periodate (Brucine)
Author(s): Venkata Rao Basa and Srinivasa Rao Tirunagari
Abstract: Background: A rapid and easy to perform method for the quantitative determination of Metoprolol tartrate (MPT) in biological matrices would be useful in its therapeutic monitoring as well as in intoxication cases. The study presents a validated method for the UV-VIS quantitative assay of MPT in bulk and tablet formulations at λmax of 530 n.m. with molar absorptivity of 1.65 × 103 L. mole-1 cm-1.
Experimental: The parameters targeted for the validation were: linearity, sensitivity, accuracy and precision. The presence of secondary amine group in MPT molecule is the basis for the proposed visible spectrophotometric method. Brucine (2, 3- dimethoxy stricychnidin-10-one) has been proved to be a valuable chromogenic reagent for the detection and determination of amino compounds.
Results: This method involves the reaction of Metoprolol with Brucine in the presence of IO4-. Periodate converts Brucine to bruciquinone, which in turn undergoes Nucleophilic attack by the most electron rich position of the coupler aliphatic primary or secondary amine to yield 1- mono substituted bruciquinone derivatives with an absorption maximum at 510-530 nm.
Conclusion: The developed method is more selective, sensitive, reproducible, rapid, cheap and simple than in most of the analogous methods. For these reasons, it can be used in routine analysis and can be applied for the determination of MPT in pharmaceutical formulations and biological samples.
DOI: 10.22271/reschem.2024.v5.i2a.142
Pages: 45-51 | Views: 131 | Downloads: 72
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How to cite this article:
Venkata Rao Basa, Srinivasa Rao Tirunagari. Development and validation of spectrophotometric method for assay of Di[RS]-3-[4-(2-methoxy ethyl)phenoxy-1-(Isopropyl amino) propan-2-ol] tartrate (Metoprolol tartrate) by using 2, 3- dimethoxy stricychnidin-10-one-Periodate (Brucine). J Res Chem 2024;5(2):45-51. DOI: 10.22271/reschem.2024.v5.i2a.142