2025, Vol. 6, Issue 1, Part B
Synthesis and characterization of Coffea arabica nanoparticles: Evaluation of antioxidant and anticancer properties
Author(s): Anil Kumar, Arpna and Sunil Kumar Jangra
Abstract: In recent years, the development of nanoparticles using natural plant extracts has gained significant attention due to their potential biomedical applications, including antioxidant and anticancer properties. This study aims to synthesize and characterize nanoparticles derived from Coffea arabica extracts using an eco-friendly, green chemistry approach. The process was optimized to produce stable nanoparticles without the use of harmful chemicals, thus promoting sustainable practices in nanomaterial synthesis. The synthesized nanoparticles were subjected to a thorough characterization using a combination of advanced techniques, including Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), and UV-Vis spectroscopy to determine their size, morphology, crystalline nature, and functional groups. These analyses revealed well- dispersed, spherical nanoparticles with an average size in the range of 50-100 nm, exhibiting good stability.
The antioxidant potential of Coffea arabica nanoparticles was evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay, which demonstrated significant free radical scavenging activity, indicative of their strong antioxidant capacity. Additionally, the anticancer activity was investigated against selected cancer cell lines, including human breast cancer (MCF-7) and colon cancer (HT-29) cell lines, using the MTT (3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The nanoparticles exhibited dose-dependent cytotoxicity, with notable inhibition of cancer cell proliferation. The findings of this study suggest that Coffea arabica nanoparticles possess substantial antioxidant properties and anticancer potential, positioning them as promising candidates for further exploration in biomedical applications, particularly in cancer therapy. The green synthesis approach employed here also highlights the environmental and economic advantages of utilizing natural plant sources for nanoparticle fabrication.
Pages: 118-123 | Views: 50 | Downloads: 29
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How to cite this article:
Anil Kumar, Arpna, Sunil Kumar Jangra. Synthesis and characterization of Coffea arabica nanoparticles: Evaluation of antioxidant and anticancer properties. J Res Chem 2025;6(1):118-123.
