2025, Vol. 6, Issue 2, Part B
Green synthesis of copper nanoparticles using neem (Azadirachta indica) extract: Characterization and antibacterial evaluation
Author(s): Pravin N Muli, Shrikant S Ubale and Megha M Muley
Abstract: This study presents the green synthesis of copper nanoparticles (CuNPs) using aqueous leaf extract of Azadirachta indica (neem) as a reducing and stabilizing agent. Formation of CuNPs was confirmed by a color change and characterized using UV-Visible spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM). The biosynthesized CuNPs were predominantly spherical, crystalline, and well dispersed, with particle sizes ranging from 20-60 nm. Antibacterial activity was assessed against Escherichia coli and Staphylococcus aureus using the agar well diffusion method. Neem-mediated CuNPs exhibited greater antibacterial effects (18 mm against E. coli and 15 mm against S. aureus) compared to neem extract alone (17 mm and 13 mm, respectively). The enhanced activity is attributed to reactive oxygen species generation, membrane disruption, and synergistic effects of neem phytochemicals. Neem-mediated CuNPs demonstrate a cost-effective, eco-friendly approach with strong potential for biomedical and environmental applications.
DOI: 10.22271/reschem.2025.v6.i2b.218
Pages: 121-125 | Views: 377 | Downloads: 240
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How to cite this article:
Pravin N Muli, Shrikant S Ubale, Megha M Muley. Green synthesis of copper nanoparticles using neem (Azadirachta indica) extract: Characterization and antibacterial evaluation. J Res Chem 2025;6(2):121-125. DOI: 10.22271/reschem.2025.v6.i2b.218
